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A Biosensor Using Poly(4-Aminophenol)/acetylcholinesterase modified graphite electrode for the detection of dichlorvos BABT
Melo,Edmar Isaías; Franco,Diego Leoni; Afonso,André Santiago; Rezende,Hélen Cristine; Brito-Madurro,Ana Graci; Madurro,João Marcos; Coelho,Nívia Maria Melo.
The properties of poly(4-aminophenol) modified graphite electrode as material for the immobilization of acetylcholinesterase were investigated by the Cyclic Voltammetry, Electrochemical Impedance Spectroscopy and Atomic Force Microscopy. The polymer was deposited on graphite electrode surface by the oxidation of 4-aminophenol and then acetylcholinesterase was immobilized on the surface of the electrode. The biosensor coupled in the continuous flow system was employed for the detection of dichlorvos. The detection and quantification limits were 0.8 and 2.4 μmol L-1 dichlorvos, respectively. Graphite electrodes modified with the poly(4-aminophenol) showed to be an efficient and promising material for immobilization of acetylcholinesterase enzyme. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Graphite; Poly(4-aminophenol); Biosensor; Dichlorvos.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600018
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Histopathological changes in the gill and liver tissues of freshwater fish, Cirrhinus mrigala exposed to dichlorvos BABT
Velmurugan,Babu; Selvanayagam,Mariadoss; Cengiz,Elif Ipek; Unlu,Erhan.
The histopathological effects of dichlorvos, an organophosphate pesticide, on the gill and liver tissues in Cirrhinus mrigala were determined by light microscopy. The fishes were exposed to sublethal concentrations (0.91 and 1.82 ppm) of dichlorvos for 10 days with parallel untreated control. No histopathological effects were observed in control group. Hyperplasia, desquamation, and necrosis of epithelial, epithelial lifting, oedema, lamellar fusion, collapsed secondary lamellae, curling of secondary lamellae and aneurism in the secondary lamellae were observed in gill tissues exposed to dichlorvos. Hepatic lesions in the liver tissues of fishes exposed to dichlorvos were characterized by cloudy swelling of hepatocytes, congestion, vacuolar degeneration,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dichlorvos; Organophosphate pesticide; Cirrhinus mrigala; Histopathology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500029
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Physical constraints of bath treatments of Atlantic salmon (Salmo salar) with a sea lice burden (Copepoda: Caligidae) Naturalis
Treasurer, James W.; Grant, Andrew; Davis, Paul J..
Licensed medicines available in the U.K. for treating Atlantic salmon infested with sea lice, dichlorvos, azamethiphos, and hydrogen peroxide, can only be administered by bath application. Adverse reactions have been reported to bath treatments including mortalities, inappetance, reduction in growth and reduced louse sensitivity to dichlorvos. The physical constraints of bath treatments are examined and improvements recommended. Oxygen saturation was adequate during treatments but declined rapidly when the tarpaulin was removed. A chemical marker dispersed uniformally both horizontally and vertically in a cage within 5 mins of dispensing indicating dispersal of a medicine is rapid and adequate during treatment. The range in enclosed volumes in 86...
Tipo: Article / Letter to the editor Palavras-chave: Sea lice; Atlantic salmon; Salar; Bath application; Dichlorvos; Hydrogen peroxide.
Ano: 2000 URL: http://www.repository.naturalis.nl/record/534338
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Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro Biol. Res.
Eroglu,Sema; Pandir,Dilek; Uzun,Fatma G; Bas,Hatice.
Organophosphate (OP) pesticides such as dichlorvos (DDVP) intoxication has been shown to produce oxidative stress due to the generation of free radicals, which alter the antioxidant defense system in erythrocytes. In this study, the effects of DDVP (1, 10, 100 µM) or DDVP + vitamin C (VC; 10 µM) or vitamin E (VE; 30 µM), on the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes were examined in vitro. There were no statistical differences between all groups for 1 µM concentration of DDVP. Treatment with DDVP alone produced an increase in the level of MDA and decreased activities of antioxidant enzymes (P < 0.05). Groups treated with vitamins and DDVP showed...
Tipo: Journal article Palavras-chave: Antioxidant enzyme; Dichlorvos; Oxidative stress; Vitamin; Malondialdehyde.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100005
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